Fig. 5

circMED13L_012 modulates miR-433-3p through affecting MAPK8. a miR-433-3p target gene prediction based on three microRNA-gene interaction databases including DIANA, miRDB and TargetScan. b–f qRT-PCR study exploring the association of miR-433-3p expression level and MAPK8 (B), CCDC138 (C), B4GALT3 (D), CEP135 (E), TOP1 (F) mRNA expression level in NSCLC clinical samples. g Prediction of miR-433-3p binding site with wildtype MAPK8 3’UTR region and design of vector including mutated MAPK8 3’UTR region. h qRT-PCT validation of the impact of miR-433-3p specific mimics and inhibitor on miR-433-3p expression. i, j Dual luciferase reporter gene assay to evaluate modulative effects of miR-433-3p specific inhibitor (I) and mimics (J) on MAPK8 expression. k. Western blot analysis on the modulative effects of miR-433-3p specific inhibitor and mimics on the MAPK8 protein expression level