Fig. 4

CircMMP11 increased ZEB2 expression through sponging miR-625-5p. a Schematic illustration showed the overlap of the target miRNAs of circMMP11 predicted by Circbank, Starbase, and Circular RNA interactome. b, c The relative levels of miRNA candidates in MCF-7 and MDA-MB-231 cells lysates were examined by RT-qPCR after pulling down by circMMP11 probe. d Predicted binding regions between circMMP11 and miR-625-5p were shown. e The binding sites for miR-625-5p in 3′UTR of ZEB2 were presented. f–k The interaction relationships among circMMP11, miR-625-5p, and ZEB2 were confirmed by dual-luciferase report and RNA pull-down assays. l, m The expression level of miR-625-5p was measured by RT-qPCR assay in breast cancer tissues and cells, along with in matched controls. ***P < 0.001