Fig. 5

Knockdown of circMMP11-mediated effects on breast cancer cells could be abolished by overexpression of ZEB2. a, b The overexpression effectiveness of ZEB2 was checked by RT-qPCR and western blot assays. c–l MCF-7 and MDA-MB-231 cells were transfected with si-NC, si-circMMP11#2, si-circMMP11#2 + pcDNA, or si-circMMP11#2 + ZEB2. c, d The expression levels of ZEB2 were assessed by RT-qPCR and western blot assays. e–g The proliferation was examined by MTT and colony-forming assays in MCF-7 and MDA-MB-231 cells. h, i The migration and invasion were determined by transwell assay. j The flow cytometry assay was performed in MCF-7 and MDA-MB-231 cells. i–k The protein expression levels of E-cadherin and Vimentin were measured by western blot assay in MCF-7 and MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001