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Fig. 5 | Cancer Cell International

Fig. 5

From: CircMMP11 regulates proliferation, migration, invasion, and apoptosis of breast cancer cells through miR-625-5p/ZEB2 axis

Fig. 5

Knockdown of circMMP11-mediated effects on breast cancer cells could be abolished by overexpression of ZEB2. a, b The overexpression effectiveness of ZEB2 was checked by RT-qPCR and western blot assays. cl MCF-7 and MDA-MB-231 cells were transfected with si-NC, si-circMMP11#2, si-circMMP11#2 + pcDNA, or si-circMMP11#2 + ZEB2. c, d The expression levels of ZEB2 were assessed by RT-qPCR and western blot assays. eg The proliferation was examined by MTT and colony-forming assays in MCF-7 and MDA-MB-231 cells. h, i The migration and invasion were determined by transwell assay. j The flow cytometry assay was performed in MCF-7 and MDA-MB-231 cells. ik The protein expression levels of E-cadherin and Vimentin were measured by western blot assay in MCF-7 and MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001

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