Fig. 1

LINC00221 is robustly induced in HCC. a heat maps with hierarchical clustering analysis for microarray GSE33006 obtained from GEO database (http://www.ncbi.nlm.nih.gov/geo). The abscissa indicates different individuals, the ordinate on the right indicates the gene; the left dendrogram refers to clustering. The upper figure shows whether the sample type consists of normal or tumor; the histogram in the upper right refers to color gradation, which indicates gene expression. The color refers to the analysis of DEGs expression, with each square representing the expression of one gene in one sample. b Circos map of microarray GSE33006. c LINC00221 expression in HCC from TCGA database (http://cancergenome.nih.gov/). d LINC00221 expression in the HCC tissues and adjacent non-tumor tissues examined by RT-qPCR (n = 45). e the expression of LINC00221 in 3 MHCC97-H, Huh7 and Hep3B cell lines determined by RT-qPCR. f Silencing efficiency of LINC00221 examined by RT-qPCR. Cell experiments were independently repeated 3 times. *p < 0.05; **p < 0.05. Comparison between two groups was made by paired t-test while comparison among multiple groups was made using one-way ANOVA with Tukey’s post hoc test