Fig. 4

Exosomal LINC00470 in serum of glioma patients mediated the autophagy and proliferation of glioma cells. Note: U251 and SWO-38 cells were firstly incubated with GBM-exo or HC-exo and transfected with sh-LINC00470 before the regulatory role of LINC00470 in exosome on glioma cell was assessed. Expression of LINC00470 was detected by qRT-PCR (a). Number of acidic autophagosomes was shown through MDC staining (b). Western blotting analyzed autophagy-related proteins expression levels including LC3-II/LC3-I, Beclin1 and p62 (c). Cell proliferation was examined by CCK8 (d) and clone formation assay (e) respectively. Cell cycle was revealed by FCM (f). RNA and protein expression levels of miR-580-3p and WEE1 were detected by qRT-PCR (g) and Western blotting (h) respectively. * P < 0.05, ** P < 0.01, ***P < 0.001, compared to HC-exo group. # P < 0.05, ## P < 0.01, ###P < 0.001, compared to GBM-exo group. GBM-exo, exosomes derived from patients with glioma; HC, health checker; MDC, monodansylcadaverin staining; FCM, flow cytometry