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Fig. 5 | Cancer Cell International

Fig. 5

From: Long non-coding RNA LINC00470 in serum derived exosome: a critical regulator for proliferation and autophagy in glioma cells

Fig. 5

Exosomal LINC00470 promoted the proliferation of glioma cells through regulating autophagy. Note: U251 and SWO-38 cells were incubated with GBM-exo or transfected with pcDNA3.1-LINC00470 or treated with Rapa. MDC staining was used to detect acidic autophagosomes (a). Detection of the expression of autophagy-related proteins, LC3-I, Beclin1 and p62 was conducted by Western blotting (b). Proliferation was examined through CCK8 and clone formation assay (cd). FCM examined the effect of LINC00470 and autophagy on cell cycle of glioma cells (e). * P < 0.05, ** P < 0.01, *** P < 0.001, compared to Control group. # P < 0.05, ## P < 0.01, compared to pcDNA3.1-LINC00470 group. GBM-exo, exosomes derived from patients with glioma; MDC, monodansylcadaverin staining; FCM, Flow cytometry

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