Fig. 4

miR-29a-3p is a direct HOXA-AS3 target in GC. a, b Levels of HOXA-AS3 in the cytoplasm and nuclei of MKN-45 (a) and SGC-7901 (b) cells were measured via qPCR, with U6 and GAPDH as respective nuclear and cytoplasmic controls. c A negative correlation between HOXA-AS3 and miR-29a-3p expression was detected via qPCR in 82 GC patient tumor samples. d A qPCR approach was utilzied to measure miR-29a-3p levels in MKN-45 and SGC-7901 cells following HOXA-AS3 or control construct transfection. e Putative HOXA-AS3 sequence complementarity with miR-29a-3p, with mutated residues in reporter constructs being shown. f miR-29a-3p levels were quantified in GC cells following miR-29a-3p mimic or inhibitor transfection. g, h The impact of miR-29a-3p overexpression on WT or MUT HOXA-AS3 luciferase activity was quantified in MKN-45 (h) and SGC-7901 (i) cells. Error bars: mean ± SD from triplicate experiments. ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. NS: no significance