Fig. 2

Identification and enrichment analysis of DEGs related to anti-EGFR antibody resistance in CACO2. a Establishment of a cetuximab-resistant CACO2 cell line (CACO2-CR) from its parental sensitive cell line (CACO2-CS) and validation of the resistance using cell viability tests. b Comparison of total gene expression between duplicate samples from the innate sensitive group (blue) and adaptive resistant (red) group. c Principal component analysis results suggested favorable reproducibility between the sensitive (blue) and resistant (red) groups. d The volcanic map reveals the distributions of downregulated (blue) and upregulated (orange) genes in the resistant group versus the sensitive group. Top 10 of DEGs between two groups were shown. e The heat map indicates the upregulated (red) and downregulated (blue) DEGs between the sensitive (blue) and resistant (red) groups. Each column is a sample and each row is a gene. Top 10 of DEGs between two groups were shown. f GO biological process term enrichment analysis of DEGs (left) and their interrelationships (right). g GO molecular function term enrichment analysis of DEGs (left) and their interrelationships (right). h GO cellular component term enrichment analysis of DEGs (left) and their interrelationships (right). i KEGG pathway enrichment analysis of DEGs (left) and their interrelationships (right). DEGs: Differentially expressed genes. GO: Gene Ontology. KEGG: Kyoto Encyclopedia of Genes and Genomes