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Fig. 8 | Cancer Cell International

Fig. 8

From: Systematic analysis of the expression and prognosis relevance of FBXO family reveals the significance of FBXO1 in human breast cancer

Fig. 8

The knockdown of FBXO1 attenuates the proliferation and migration of breast cancer cells in vitro. a Upper panel, the expression levels of FBXO1 protein examined by Western blotting in 8 human breast cell lines. Lower panel, bar graphs representing quantification of Western blotting bands. b Determination of relative mRNA expression levels of FBXO1 in control and si‐FBXO1‐transfected MCF7 and MDA-MB-231 breast cancer cell lines by RT-qPCR assay. c Immunoblotting analyses of proteins as indicated in control and si‐FBXO1‐transfected MCF7 and MDA-MB-231 cell lines, bar graphs representing quantification of Western blotting bands. d Diagram of successful transfection of siRNA of FBXO1 labeled by FAM fluorescence dye in MCF7 and MDA-MB-231 cell lines. e The knockdown of FBXO1 attenuates the proliferation of breast cancer cells in vitro. Cell Counting Kit-8 assay showed the relative proliferative capacity of specific MCF7 and MDA-MB-231 cells at 24, 48, and 72 h after seeding in plates. f The knockdown of FBXO1 attenuates the proliferation of breast cancer cells in vitro. Colony-forming assay showed the relative proliferative capacity of specific MCF7 and MDA-MB-231 cells at 48 h after seeding in plates(left) and quantification of the colony areas (right). g The knockdown of FBXO1 attenuates the migration of breast cancer cells in vitro. Transwell migration assay showed representative images of specific MCF7 and MDA-MB-231 cells (left) and quantification of the cell numbers (right). h The knockdown of FBXO1 attenuates the migration of breast cancer cells in vitro. Wound‐healing assay for MCF7 and MDA-MB-231 and wound closure was monitored at 0, 24, and 48 h. Data in bar graphs are the means ± SD of three independent experiments. **P < 0.01; ***P < 0 .001 by Student t test. siRNA, small interfering RNA; RT-qPCR, Real Time Quantitative Polymerase Chain Reaction

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