Fig. 8From: Systematic analysis of the expression and prognosis relevance of FBXO family reveals the significance of FBXO1 in human breast cancerThe knockdown of FBXO1 attenuates the proliferation and migration of breast cancer cells in vitro. a Upper panel, the expression levels of FBXO1 protein examined by Western blotting in 8 human breast cell lines. Lower panel, bar graphs representing quantification of Western blotting bands. b Determination of relative mRNA expression levels of FBXO1 in control and si‐FBXO1‐transfected MCF7 and MDA-MB-231 breast cancer cell lines by RT-qPCR assay. c Immunoblotting analyses of proteins as indicated in control and si‐FBXO1‐transfected MCF7 and MDA-MB-231 cell lines, bar graphs representing quantification of Western blotting bands. d Diagram of successful transfection of siRNA of FBXO1 labeled by FAM fluorescence dye in MCF7 and MDA-MB-231 cell lines. e The knockdown of FBXO1 attenuates the proliferation of breast cancer cells in vitro. Cell Counting Kit-8 assay showed the relative proliferative capacity of specific MCF7 and MDA-MB-231 cells at 24, 48, and 72 h after seeding in plates. f The knockdown of FBXO1 attenuates the proliferation of breast cancer cells in vitro. Colony-forming assay showed the relative proliferative capacity of specific MCF7 and MDA-MB-231 cells at 48 h after seeding in plates(left) and quantification of the colony areas (right). g The knockdown of FBXO1 attenuates the migration of breast cancer cells in vitro. Transwell migration assay showed representative images of specific MCF7 and MDA-MB-231 cells (left) and quantification of the cell numbers (right). h The knockdown of FBXO1 attenuates the migration of breast cancer cells in vitro. Wound‐healing assay for MCF7 and MDA-MB-231 and wound closure was monitored at 0, 24, and 48 h. Data in bar graphs are the means ± SD of three independent experiments. **P < 0.01; ***P < 0 .001 by Student t test. siRNA, small interfering RNA; RT-qPCR, Real Time Quantitative Polymerase Chain ReactionBack to article page