Fig. 3

SNHG6 functioned as a sponge of miR-6509-5p. a The illustration of the luciferase assay to test the binding between SNHG6 and miR-6509-5p. b SNHG6-WT or SNHG6-MUT was co-transfected with miR-6509-5p, and the luciferase activities of SNHG6 reporter vectors were measured. c The binding between SNHG6 and miR-6509-5p was further confirmed using RNA immunoprecipitation (RIP) with the anti-AGO2 antibody. d The relative expression level of miR-6509-5p was detected using Hep3B and Huh7 cells. e The expression level of miR-6509-5p in HCC patients was measured. f, g The proliferation ability of HCC cell lines after up-regulation of miR-6509-5p with miR-6509-5p mimics was detected using EdU labeling, and h EdU positive cells per field were calculated. i, j The migration and invasion abilities of HCC cell lines after up-regulation of miR-6509-5p were evaluated using the transwell assay. k The percent of Huh7 cells in each cell cycles after the treatment of miR-6509-5p. l The apoptosis level of Huh7 cells after the treatment of miR-6509-5p. *P < 0.05, **P < 0.01, ***P < 0.001. Data were presented as mean ± SD from 6 independent experiments