Fig. 4

The expression of HIF1A was regulated by SNHG6/miR-6509-5p axis. a The illustration of the luciferase assay to test the binding between miR-6509-5p and HIF1A. b Luciferase activity of miR-6509-5p was measured to test the binding effect between miR-6509-5p and HIF1A. c, d The expression levels of HIF1A in Hep3B and Huh7 cells after treating with up- or down-regulation of miR-6509-5p were detected with quantitative PCR. e The levels of HIF1A in Hep3B and Huh7 cells after treating with up- or down-regulation of miR-6509-5p were detected with WB. f The migration and invasion abilities of Huh7 cells after up-regulation of HIF1A, and miR-6509-5p mimics reversed the effect. g The apoptosis level of Huh7 cells after up-regulation of HIF1A, and miR-6509-5p mimics reversed the effect. *P < 0.05, **P < 0.01, ***P < 0.001. Data were presented as mean ± SD from 3 independent experiments