Fig. 4

GM-CSF enhances the effect of CHOP and R-CHOP on the polarization of M1 macrophages in vitro. a PMA treatment induced the differentiation of THP-1 to M0 macrophages. b M0 macrophages were stimulated with IL-4 and IL-13, flow cytometry was carried out to detect the expression of CD163 on THP-1 cells. c ELISA was used to detect the IL-12 p70 level of M0 and M2 cell. d ELISA was used to detect the IL-10 level of M2 cells. (A)**p < 0.01 vs. THP-1, (B)**p < 0.01 vs. THP-1 + PMA, (c and d) **p < 0.01 vs. Control, ##p < 0.01 vs. CHOP, @@p < 0.01 vs. CHOP + GM-CSF, $$p < 0.01 vs. R-CHOP. Data are representative of at least three independent experiments