Fig. 5

Effects of VIM-AS1 and miR-655 on the growth of xenograft formed in nude mice. The stable T24 cells of Lv-negative control (NC), Lv-VIM-AS1, Lv-miR-655 and Lv-VIM-AS1 + miR-655 were subcutaneous injected into the armpit of nude mice respectively. a At the end of the xenograft formed experiment (the 25th day), mice were euthanized and tumor tissues were excised, the tumor volumes, weight and sizes in different experiment groups were detected. b IHC staining (×200) was applied to detect the protein expression of proliferation marker Ki67 and ZEB1 in tumor tissues from nude mice. c The VIM-AS1 and miR-655 expression levels in tumor tissues from nude mice were determined by real-time PCR assay. *P < 0.05, **P < 0.01 compared to Lv-NC group; ^##P < 0.01 compared to Lv-VIM-AS1 or Lv-miR-655 group