Table 1 The primer sequence
Name | Forward | Reverse |
|---|---|---|
RT-PCR VIM-AS1 | CTCAGACCTGTAGCATCAGCATCAC | TTCATTTCCTCATAAGCGAACACTC |
RT-PCR ZEB1 | AGCAGTGAAAGAGAAGGGAATGC | GGTCCTCTTCAGGTGCCTCAG |
RT-PCR Vimentin | AGTCCACTGAGTACCGGAGAC | CATTTCACGCATCTGGCGTTC |
RT-PCR GAPDH | ACAGCCTCAAGATCATCAGC | GGTCATGAGTCCTTCCACGAT |
RT-PCR miR-655-3p | RT:GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAAAGAG F: GCCGCATAATACATGGTTAAC | CAGTGCGTGTCGTGGA |
RT-PCR U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
NC mimics | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
NC inhibitor | CAGUACUUUUGUGUAGUACAA | |
MiR-655 mimics | AUAAUACAUGGUUAACCUCUUU | AGAGGUUAACCAUGUAUUAUUU |
MiR-655 inhibitor | AAAGAGGUUAACCAUGUAUUAU | |
Si-VIM-AS1 1# | GGAUGUGUAAGAGAAUUUATT | UAAAUUCUCUUACACAUCCTT |
Si-VIM-AS1 2# | CCAUGUGUGCGAUUCACAATT | UUGUGAAUCGCACACAUGGTT |
Si-ZEB1 | CCUAGUCAGCCACCUUUAATT | UUAAAGGUGGCUGACUAGGTT |
Si-NC | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
Wt-VIM-AS1 | aattctaggcgatcgctcgagAGATTTTAAAATAGAGCATGCTTAATATACTT | attttattgcggccagcggccgcCACTAGTACACCCCCGACGTG |
Mut-VIM-AS1 | AGTAtgcgctGTATTGGCTGTCCCACATTTTGA | GCCAATACagcgcaTACTACTAAGTAATAAATCTATGTCATCCAAAGG |
Wt-ZEB1 3′UTR | aattctaggcgatcgctcgagATTTGGCTCATAACTGTTTCCAAA | attttattgcggccagcggccgcTTTCATACTAAAATATATTATTGTATTAATACAAACTAC |
Mut-ZEB1 3′UTR | AgcgcgcaTACCCTTCTTACTGACATATGTACTTTTAGTT | AGAAGGGTAtgcgcgcTGCCAGGTTTGAAGACATACAGTAC |
Lv-VIM-AS1 overexpression | ctaccggactcagatctcgagTTCTCCCGGAGGCGCATG | gtaccgtcgactgcagaattcTTTTTTTTTAGCATATCCAAAAATGG |
Lv-miR-655-3p overexpression | ctaccggactcagatctcgagATATTTTTAATGGAACCTGCCTTAGA | gtaccgtcgactgcagaattcACAGCAAACACAGCAAAGCAAC |
ZEB1 overexpression | ctaccggactcagatctcgagATGAAAGTTACAAATTATAATAC | gtaccgtcgactgcagaattcTTAGGCTTCATTTGTCTTTTC |