Fig. 4

Determination of MSCs migration potential following treatment with oncolytic reovirus in vitro. a Representative images of cell migration in the scratch assay of MSCs with or without reovirus infection through different MOI 0 (Control), 1, 5, and 10 in 24 h P.I. There were many migrated cells in the scratched space after 24 h depended on cell integrity. Images were captured in triplicate for precise analysis. b After 24 h P.I, the wound healing rates were assessed. Images were captured in triplicate for precise analysis. Macroscopic size of wound healing rates was calculated using ImageJ software. Data were calculated as mean ± SD. In the first 24 h P.I, the relative reduction in wound healing was assessed. Abrupt activation of NF-κB via reovirus infection can trigger the expression of a wide range of genes that are engaged in the cell migration process. The high MOI [10] damage cell integrity that subsequently has an adverse effect on cell mobility. c The migration potency of infected MSCs was analyzed by the evaluation of surface CXCR4 marker in variant MOI (control, 1, 5, and 10) and time (24, 48, and 72 h P.I) by flow cytometry. In MOI 10 reovirus showed a destructive effect on MSCs and declined results on migration capacity (P-value: 0.0106). This experiment was repeated three times and statistically, the analysis was reported by Tukey's post hoc test. (***: P ≤ 0.001)