Fig. 9

a The in vivo distribution of MSCs were traced in mice organs and b virus genome detection in tumors site. a After first accumulation in the lung, the I.V injected CFSE-Labelled MSCs were distributed in mice's body via blood circulating system and additionally in the tumor sites. Released soluble factors such as chemokines, cytokines, and growth factors from tumor cells could recruit MSCs to tumor sites (b). The results of PCR showed that the MSCs could act as a delivery vehicle to the tumor site. The size of the amplified L1 segment of reovirus was 135 bp length. To visualize the PCR product 2% agarose gel electrophoresis is used.