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Table 4 Specifications of all antibodies used in AD-MSCs immunophenotyping and cytokine assays

From: MSCs loaded with oncolytic reovirus: migration and in vivo virus delivery potential for evaluating anti-cancer effect in tumor-bearing C57BL/6 mice

Antibody

Catalogue no

Company

Dilutions titer

Mouse IFN-gamma DuoSet ELISA

DY485-05

R & D systems

Capture 4 µg/ml

Detection 400 ng/ml

Mouse IL-10 DuoSet ELISA

DY417-05

R & D systems

Capture 4 µg/ml

Detection 300 ng/ml

Mouse TGF-β DuoSet ELISA

DY402-05

R & D systems

Capture 4 µg/ml

Detection 75 ng/ml

Mouse IL-6 DuoSet ELISA

DY406-05

R & D systems

Capture 2 µg/ml

Detection 50 ng/ml

Anti- mouse CD34-FITC

11-0341-82

e-Bioscience

1 µg/test

Anti- mouse CD45- FITC

11-0451-82

e-Bioscience

0.5 µg/test

Anti- mouse CD29-PE

12-0291-82

e-Bioscience

1 µg/test

Anti- mouse CD73 -PE

12-0731-82

e-Bioscience

0.125 µg/test

Anti- mouse CD90-PE

12-0902-82

e-Bioscience

0.5 µg/test

Rat IgG2a kappa Isotype Control- FITC

11-4321-42

e-Bioscience

1 µg/test

Rat IgG2b kappa Isotype Control- FITC

11-4031-85

e-Bioscience

1 µg/test

Rat IgG1 Isotype Control—PE

12-4301-82

e-Bioscience

0.125 µg/test

Rat IgG2a kappa Isotype Control -PE

12-4321-80

e-Bioscience

0.5 µg/test