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Fig. 4 | Cancer Cell International

Fig. 4

From: The loss of DLG2 isoform 7/8, but not isoform 2, is critical in advanced staged neuroblastoma

Fig. 4

LIN7A expression is affected by DLG2-isoform 7. a The Relationship of DLG2-isoform 7/8 and LIN7A expression in 22 NB primary tumor samples. The relative mRNA expression of DLG2 and LIN7A for each sample is determined. The data expressed as relative log2 fold change after normalization to GAPDH and GUSB with linearity determined using a line of best fit, created with a Deming (Model II) regression. b LIN7A gene expression 48 h post DLG2-isoform 7 over expression (DLG2-iso7-DYK) or silencing (si-DLG2) in SKNAS cells. c. Western blot of SKNAS transfected cells 48 h post DLG2-isoform 7 over expression (DLG2-iso7-DYK) or silencing (si-DLG2) quantifying DLG2, LIN7A and GAPDH expression. d DLG2 gene expression 48 h post LIN7A over expression (LIN7A-GFP) or silencing (si-LIN7A) in SKNAS cells. e Co-immunoprecipitation of HEK293 cells co-transfected with DLG2-iso7-DYK and LIN7A-GFP or DLG2-iso2-DYK and LIN7A-GFP plasmids. Detection of the lysate with DYK or LIN7A antibody. f Cell cycle analysis after DLG2-iso2 or DLG2-iso7 over expression. The data in b and d are shown as the mean ± SD. The data in (eh) are presented as Tukeys box and whisker plots showing IQR, line at the median, + at the mean with whiskers ± 1.5-fold of interquartile range ***p < 0.001, ns = not significant

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