Fig. 6
From: Challenges of applying multicellular tumor spheroids in preclinical phase
Copyright 2017, Elsevier. (b) A schematic diagram of the pneumatic microstructure array and its operating principle [141]. Copyright 2015, The Royal Society of Chemistry. (c) A schematic diagram of the microfluidic pillar array with cell seeding and collection processes [143]. Copyright 2018, The Royal Society of Chemistry. (d) Schematic and optical images of droplet-based microfluidic systems for MCT fabrication [53]. Copyright 2018, Elsevier. B High-throughput drug screening. (a) Microfluidic device for rapid tumor spheroid growth consisting of a semi-permeable polycarbonate membrane [52]. Copyright 2019, The Royal Society of Chemistry. (b) The microfluidic device generates a concentration gradient of fluorescein isothiocyanate (FITC). The fluorescent images of channels and concave microwells (C1–C5) were taken 16 h after an injection of phosphate-buffered saline (PBS) and FITC [133]. Copyright 2018, Permits unrestricted use. (c). The architecture and operation of the software-programmable microfluidic platform [154]. Copyright 2018, The Royal Society of Chemistry
A MCTs generation in a microfluidic device. (a) Schematics of a microchip containing of 4 rows of microchambers that contain 7 microwells [130].