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Table. 1. MCTs formation depends on the cell type

From: Challenges of applying multicellular tumor spheroids in preclinical phase

Tumor type Cell line MCTs morphology Culture conditions (media, peroid, technology) References
Breast cancer MCF-7 CS RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate [22]
BT-474 CS Sodium pyruvate + HEPES, 24 h, low adhesion plate
T-47D CS RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate
MDA-MB-361 CS RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate
MDA-MB-435S TA RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate
MDA-MB-231 LA RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate
MDA-MB-468 LA RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate
SK-BR-3 LA McCoy's 5A, 24 h, low adhesion plate
MCF-7 CS RPMI + 25% methocel, 3 days, hanging drop [4]
MDA-MB-231 TA RPMI + 25% methocel, 3 days, hanging drop
SK-BR-3 LA RPMI + 25% methocel, 3 days, hanging drop
Colon cancer HCT116 CS High glucose, 4 days culture low adhesion plate [39]
DLD-1 TA DMEM, 4 days culture low adhesion plate
SW620 LA RPMI, 4 days, low adhesion plate
Gastric cancer RF-1 CS RPMI, 2 days, liquid overlay technique [38]
RF-48 CS RPMI, 2 days, liquid overlay technique
Hs-746 T CS RPMI, 2 days, liquid overlay technique
MKN-28 TA RPMI, 2 days, liquid overlay technique
MKN-74 TA RPMI, 2 days, liquid overlay technique
N87 TA RPMI, 2 days, liquid overlay technique
SNU-5 LA RPMI, 2 days, liquid overlay technique
SNU-16 LA RPMI, 2 days, liquid overlay technique
Head and neck cancer FaDu CS RPMI + 10% FCS, 72 h, low adhesion plate [40]
HLaC78 CS RPMI, 2 days, liquid overlay technique
HSmC78 CS RPMI, 2 days, liquid overlay technique
Cal27 CS RPMI, 2 days, liquid overlay technique
PE/CA-PJ41 CS RPMI, 2 days, liquid overlay technique
HNO210 CS DMEM, 72 h, low adhesion plate
SCC4 CS DMEM/F12, 72 h, low adhesion plate
FaDu TA RPMI, 2 days, liquid overlay technique
Hep2 TA MEM, 72 h, low adhesion plate
HPaC79 LA RPMI, 2 days, liquid overlay technique
HLaC79 LA RPMI, 2 days, liquid overlay technique
HLaC79-tAX LA RPMI, 2 days, liquid overlay technique
Glioblastoma U-87 MG CS DMEM, 24–48 h, low adhesion plate [41]
A172 CS DMEM, 24–48 h, low adhesion plate
SF126 CS DMEM, 24–48 h, low adhesion plate
LN-229 CS DMEM, 24–48 h, low adhesion plate
Neuroblastoma SH-SY5Y CS RPMI, 24–48 h, low adhesion plate [41]
KELLY CS RPMI, 24–48 h, low adhesion plate
SHEP TA RPMI, 24–48 h, low adhesion plate
IMR-32 TA DMEM, 24–48 h, low adhesion plate
Pancreatic PANC-1 CS DMEM, 24–48 h, low adhesion plate [41]
MIA PaCa-2 LA DMEM, 24–48 h, low adhesion plate
  1. CS compact spheroids, TA tightly aggregated, LA loosely aggregated