Skip to main content

Table. 1. MCTs formation depends on the cell type

From: Challenges of applying multicellular tumor spheroids in preclinical phase

Tumor type

Cell line

MCTs morphology

Culture conditions (media, peroid, technology)

References

Breast cancer

MCF-7

CS

RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate

[22]

BT-474

CS

Sodium pyruvate + HEPES, 24 h, low adhesion plate

T-47D

CS

RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate

MDA-MB-361

CS

RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate

MDA-MB-435S

TA

RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate

MDA-MB-231

LA

RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate

MDA-MB-468

LA

RPMI + 10% FCS + L-Glutamine, 24 h, low adhesion plate

SK-BR-3

LA

McCoy's 5A, 24 h, low adhesion plate

MCF-7

CS

RPMI + 25% methocel, 3 days, hanging drop

[4]

MDA-MB-231

TA

RPMI + 25% methocel, 3 days, hanging drop

SK-BR-3

LA

RPMI + 25% methocel, 3 days, hanging drop

Colon cancer

HCT116

CS

High glucose, 4 days culture low adhesion plate

[39]

DLD-1

TA

DMEM, 4 days culture low adhesion plate

SW620

LA

RPMI, 4 days, low adhesion plate

Gastric cancer

RF-1

CS

RPMI, 2 days, liquid overlay technique

[38]

RF-48

CS

RPMI, 2 days, liquid overlay technique

Hs-746 T

CS

RPMI, 2 days, liquid overlay technique

MKN-28

TA

RPMI, 2 days, liquid overlay technique

MKN-74

TA

RPMI, 2 days, liquid overlay technique

N87

TA

RPMI, 2 days, liquid overlay technique

SNU-5

LA

RPMI, 2 days, liquid overlay technique

SNU-16

LA

RPMI, 2 days, liquid overlay technique

Head and neck cancer

FaDu

CS

RPMI + 10% FCS, 72 h, low adhesion plate

[40]

HLaC78

CS

RPMI, 2 days, liquid overlay technique

HSmC78

CS

RPMI, 2 days, liquid overlay technique

Cal27

CS

RPMI, 2 days, liquid overlay technique

PE/CA-PJ41

CS

RPMI, 2 days, liquid overlay technique

HNO210

CS

DMEM, 72 h, low adhesion plate

SCC4

CS

DMEM/F12, 72 h, low adhesion plate

FaDu

TA

RPMI, 2 days, liquid overlay technique

Hep2

TA

MEM, 72 h, low adhesion plate

HPaC79

LA

RPMI, 2 days, liquid overlay technique

HLaC79

LA

RPMI, 2 days, liquid overlay technique

HLaC79-tAX

LA

RPMI, 2 days, liquid overlay technique

Glioblastoma

U-87 MG

CS

DMEM, 24–48 h, low adhesion plate

[41]

A172

CS

DMEM, 24–48 h, low adhesion plate

SF126

CS

DMEM, 24–48 h, low adhesion plate

LN-229

CS

DMEM, 24–48 h, low adhesion plate

Neuroblastoma

SH-SY5Y

CS

RPMI, 24–48 h, low adhesion plate

[41]

KELLY

CS

RPMI, 24–48 h, low adhesion plate

SHEP

TA

RPMI, 24–48 h, low adhesion plate

IMR-32

TA

DMEM, 24–48 h, low adhesion plate

Pancreatic

PANC-1

CS

DMEM, 24–48 h, low adhesion plate

[41]

MIA PaCa-2

LA

DMEM, 24–48 h, low adhesion plate

  1. CS compact spheroids, TA tightly aggregated, LA loosely aggregated