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Table. 2. MCTs formation depends on culture methods

From: Challenges of applying multicellular tumor spheroids in preclinical phase

Tumor type Cell line Culture technology  
Agitation‐based Liquid overlay Hanging drop Suspension References
Breast cancer MCF-7 Small spheroid
High collagen content
Large spheroid
Small collagen content
Small spheroid
High collagen content
[56]
Ovarian cancer OVCAR8 Small spheroid
High collagen content
Large spheroid
Small collagen content
Small spheroid
High collagen content
[56]
Breast cancer MCF-7 Large spheroid Large or small spheroid Large or small spheroid [4]
MDA-MB-231 Loose aggregation Tight aggregation No aggregation
SK-BR-3 Loose/no aggregation Loose aggregation No aggregation
Bladder cancer RT4 Large spheroid Small spheroid [57]
Head and neck cancer Cal27 Large spheroid Small spheroid [58]
FaDu Large spheroid Small spheroid
PiCa Large spheroid Small spheroid