From: Challenges of applying multicellular tumor spheroids in preclinical phase
Tumor type | Cell line | Culture technology | ||||
---|---|---|---|---|---|---|
Agitation‐based | Liquid overlay | Hanging drop | Suspension | References | ||
Breast cancer | MCF-7 | Small spheroid High collagen content | Large spheroid Small collagen content | Small spheroid High collagen content | – | [56] |
Ovarian cancer | OVCAR8 | Small spheroid High collagen content | Large spheroid Small collagen content | Small spheroid High collagen content | – | [56] |
Breast cancer | MCF-7 | – | Large spheroid | Large or small spheroid | Large or small spheroid | [4] |
MDA-MB-231 | – | Loose aggregation | Tight aggregation | No aggregation | ||
SK-BR-3 | – | Loose/no aggregation | Loose aggregation | No aggregation | ||
Bladder cancer | RT4 | – | Large spheroid | Small spheroid | – | [57] |
Head and neck cancer | Cal27 | – | Large spheroid | Small spheroid | – | [58] |
FaDu | – | Large spheroid | Small spheroid | – | ||
PiCa | – | Large spheroid | Small spheroid | – |