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Table. 2. MCTs formation depends on culture methods

From: Challenges of applying multicellular tumor spheroids in preclinical phase

Tumor type

Cell line

Culture technology

 

Agitation‐based

Liquid overlay

Hanging drop

Suspension

References

Breast cancer

MCF-7

Small spheroid

High collagen content

Large spheroid

Small collagen content

Small spheroid

High collagen content

[56]

Ovarian cancer

OVCAR8

Small spheroid

High collagen content

Large spheroid

Small collagen content

Small spheroid

High collagen content

[56]

Breast cancer

MCF-7

Large spheroid

Large or small spheroid

Large or small spheroid

[4]

MDA-MB-231

Loose aggregation

Tight aggregation

No aggregation

SK-BR-3

Loose/no aggregation

Loose aggregation

No aggregation

Bladder cancer

RT4

Large spheroid

Small spheroid

[57]

Head and neck cancer

Cal27

Large spheroid

Small spheroid

[58]

FaDu

Large spheroid

Small spheroid

PiCa

Large spheroid

Small spheroid