Fig. 3

CircCSPP1 targets to miR-944. a Potential targets of circCSPP1 were predicted by DIANA tool. The red trace is the predicted binding sites and the blue mark represents the circCSPP1 mutation sites. b, c Relative luciferase activity was determined in both LoVo/DOX (b) and HCT116/DOX cells (c) co-transfected with miR-944 or miR-NC and circCSPP1 WT or circCSPP1 MUT. d–f RIP (d) and RNA pull down (e, f) assays were conducted to confirm the interaction between circCSPP1 and miR-944. g, h miR-944 expression was detected by qRT-PCR assay in DOX-resistant and DOX-sensitive CRC tissues (g) or DOX-resistant and normal CRC cells (h). i MiR-944 expression was measured by qRT-PCR in cells transfected with pcDNA, circCSPP1, si-NC, and si-circCSPP1, respectively. *P < 0.05