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Fig. 6 | Cancer Cell International

Fig. 6

From: Circular RNA circCSPP1 knockdown attenuates doxorubicin resistance and suppresses tumor progression of colorectal cancer via miR-944/FZD7 axis

Fig. 6

FZD7 overexpression partly reversed the effects of miR-944 on DOX-resistant CRC cells. a–j LoVo/DOX and HCT116/DOX cells were transfected with miR-NC, miR-944, miR-944 + pcDNA, or miR-944 + FZD7, respectively. a The mRNA and protein levels of FZD7 were detected in transfected LoVo/DOX and HCT116/DOX cells. b The transfected LoVo/DOX and HCT116/DOX cells were analyzed by MTT assay. IC50 of 24 h was shown. (c–e) Cell proliferation was determined by MTT assay after transfection for 0 h, 24 h, 48 h, and 72 h (c, d) or colony formation assay (e). f, g Cell migration (f) and invasion (g) were assessed using a transwell assay. h Cell apoptosis rate was analyzed with flow cytometry analysis. i, j Western blot assay was performed to detect the protein levels of MRP1, P-gp and LRP. *P < 0.05

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