Fig. 1

Slug promotes cell migration and invasion of cervical cancer cells in vitro. Slug-overexpressing (SiHa and HeLa) and Slug knockdown (CaSki) cells were identified by western blotting: a SiHa-Vec and SiHa-Slug cells; c HeLa-Vec and HeLa-Slug cells; and e CaSki-shControl and CaSki-shSlug cells. The migratory and invasive capacities of Slug-modified cells were analyzed by the transwell cell assay, and the number of migrated cells is shown (scale bar, 100 μm): b SiHa-Vec and SiHa-Slug cells; d HeLa-Vec and HeLa-Slug cells; and f CaSki-shControl and CaSki-shSlug cells. The migratory potential of Slug-modified cells was analyzed by wound-healing assays performed for 0, 24, and 48 h (scale bar, 200 μm): g SiHa-Vec and SiHa-Slug cells, and the quantitative analysis is shown; h HeLa-Vec and HeLa-Slug cells, and the quantitative analysis is shown; and i CaSki-shControl and CaSki-shSlug cells, and the quantitative analysis is shown