Fig. 3

Slug promotes distant metastasis of SiHa and HeLa cells in vivo. a SiHa-Vec and SiHa-Slug cells were injected into female BALB/c nude mice via the tail vein. Representative hematoxylin and eosin-stained images of lung sections are presented to show the metastatic nodules in tumor lesions, scale bars, 500 μm and 200 μm. b The scatter plots show the number of metastatic nodules in the lungs as the mean ± SD (n = 5). c The number of mice that bore metastatic nodules. d The expression of Slug and EpCAM was detected in mouse metastasis tumor tissues derived from SiHa-Vec and SiHa-Slug cells by using western blotting, and the quantitative analysis was shown in (e). Data were statistically analyzed with Student’s t-test, and values are shown as the mean ± SD, * p < 0.05, ** p < 0.01 vs. control