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Fig. 4 | Cancer Cell International

Fig. 4

From: Absence of EpCAM in cervical cancer cells is involved in slug induced epithelial-mesenchymal transition

Fig. 4

Rescuing EpCAM in Slug-overexpressing cells inhibits cell motility and promotes cell growth. a The potential binding site for Slug on the promoter region of EpCAM was analyzed through the Jaspar online database. b The activity of the EpCAM promoter was measured by using the dual luciferase assay and is presented as the fold change in the activity of SiHa-Slug cells versus SiHa-Vec cells. c A quantitative ChIP assay of the EpCAM promoter region in SiHa-Slug and SiHa-Vec cells is shown. The protein levels of EpCAM, β-catenin and cyclin D1 in Slug-overexpressing cells transiently transfected with an EpCAM recombinant plasmid were detected by western blotting: d SiHa-Slug cells, g HeLa-Slug cells, and j CaSki-shCtr cells. The growth potential of Slug-overexpressing cells by transient transfection with EpCAM recombinant plasmid was detected by generating growth curves and performing MTT assays: e SiHa-Slug cells, h HeLa-Slug cells and k CaSki-shCtr cells. The migratory and invasive capacities of Slug-overexpressing cells transiently transfected with an EpCAM recombinant plasmid were analyzed by the transwell cell migration assay: f SiHa-Slug cells, i HeLa-Slug cells and l CaSki-shCtr cells. Data were statistically analyzed with Student’s t-test, and data are shown as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 vs. control

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