Fig. 5

SOCS6 is a negative regulator of ESCC cell stemness. a HPV⁺ Eca109 cells were analyzed by flow cytometry after incubation with antibodies specific for CD24/CD44 for 30 min; b the statistical results are shown (n = 3); c, e Eca109 and KYSE-150 cells were also analyzed by flow cytometry for CD24/CD44 (n = 3). SOCS6 overexpression significantly decreased the population of CD24^low/CD44^high cells; d, f the statistical results are shown (n = 3); g Eca109 and KYSE-150 cells were analyzed by flow cytometry for CD271. SOCS6 significantly decreased the population of CD271⁺ Eca109 and KYSE-150 cells. h The statistical results are shown (n = 3). The data are presented as mean ± SD, *P < 0.05, **P < 0.01 and ***P < 0.001