Fig. 6

SOCS6 regulates the ubiquitylation of c-Kit. a SOCS6 and c-Kit were immunofluorescent stained and analyzed under laser scanning confocal microscopy; b the expression of c-Kit was detected by WB. In cells overexpressing SOCS6, c-Kit expression was down regulated; c in HPV⁺ Eca109 cells, c-Kit was immunoprecipitated and ubiquitin was then immunoblotted; d, e c-Kit expression in Eca109 and KYSE-150 cells were analyzed after incubation with CHX. C-Kit was downregulated only when SOCS6 was overexpressed; g, h in Eca109 and KYSE-150 cells, c-Kit was immunoprecipitated and ubiquitin was then immunoblotted. The ubiquitylation level of c-Kit was decreased when SOCS6 was overexpressed; f, i SOCS6 and c-Kit were coimmunoprecipitated followed by WB. The data are presented as mean ± SD, *P < 0.05, **P < 0.01 and ***P < 0.001