Fig. 9

2D structure of the 10 small molecule drugs and dose–response curves (DRCs) of NVP-AUY922, fostamatinib and PP-2. a 2D structure of the 10 small molecule drugs. b–d DRCs of NVP-AUY922, fostamatinib and PP-2 in six cell lines, as determined by CCK8 assays. HN4, HN6, HN30, SCC9, SCC25 and CAL27 cell lines were seeded in 96-well culture plates, and the four drugs at different concentrations were added 24 h latter. After culture for 72 h, OD values were obtained by CCK8 assay, and cell inhibition rate was calculated. The experiments were repeated three times. e IC50 values of three small molecule drugs in six HPV-negative HNSCC cell lines. IC50 values of the drugs were calculated with GraphPad Prism software, NVP-AU922 was the most effective drug, followed by fostamatinib and PP-2