Fig. 2
ANGPTL4 downregulation in A2780 and CAOV3 ovarian cancer cells through RNA interference (RNAi) technology. a Experimental strategy. A2780 or CAOV3 cells were transduced with lentiviral vectors encoding GFP and siRNA targeting ANGPTL4 (siANG) or scrambled control siRNA (Ctl) for 3 days and were then subjected to cytological profiling. Then, 1 × 107 cells were injected into the left axilla of 5- to 6-week-old female BALB/c nude mice. Thirty days after injection, mice were sacrificed to harvest the tumors for further analysis. b Immunofluorescence of green fluorescence protein (GFP) and bright field images of A2780 and CAOV3 cells confirmed the transduction efficiency of the lentiviral vectors. Scale bars: 75 μm. c Western blot and statistical graph of ANGPTL4 expression in A2780 and CAOV3 cells transduced with siANG or Ctl; n = 3 independent experiments per group. d qRT-PCR to determine the ANGPTL4 mRNA level in A2780 and CAOV3 cells transduced with lentiviral vectors; n = 3 independent experiments per group. e Western blot and statistical graph of ANGPTL4 expression in transduced A2780 and CAOV3 tumor xenograft models; n = 5 independent experiments per group. f qRT-PCR to verify ANGPTL4 mRNA expression in transduced A2780 and CAOV3 tumors; n = 5 independent experiments per group. ***p < 0.001 for the indicated comparisons. The data are presented as the mean ± SEM values. siANG, small interfering RNA targeting ANGPTL4