Fig. 3

ANGPTL4 knockdown does not alter ovarian cancer cell proliferation, migration or invasion in vitro. a Transwell assay and statistical graph of the migration and invasion of A2780 and CAOV3 cells with or without ANGPTL4 inhibition; n = 4 independent experiments per group. Scale bar: 100 μm. b Crystal violet staining of transduced A2780 and CAOV3 cells. Scale bar: 12 mm. c A2780 and CAOV3 cell proliferation was investigated by a CCK-8 assay; n = 3 independent experiments per group. d Fluorescence staining and statistical graph of 5-ethynyl-2′-deoxyuridine (EdU) incorporation in transduced ovarian cancer cells; n = 3 independent experiments per group. Scale bar: 80 μm. e Western blot of the epithelial-mesenchymal transition (EMT)-related markers slug and snail in transduced ovarian cancer cells; n = 3 independent experiments per group. ns indicates no significance. The data are presented as the mean ± SEM values