Fig. 4

Decreased ANGPTL4 expression inhibits human ovarian cancer xenograft growth in nude mice. a–c Xenograft tumors of nude mice 30 days after injection of transduced A2780 or CAOV3 cells; n = 3 independent experiments per group. Scale bar: 7 mm. Total A2780 and CAOV3 tumor volumes were determined every 5 days. d GFP and CD31 immunofluorescence staining of A2780 and CAOV3 tumors (left) and skeletonization of CD31 staining to quantify total vessel length in the tumor vasculature in each field (right). Quantification of vessel length according to skeletonization of CD31 staining; n = 3 independent experiments per group. Scale bar: 100 μm. e Immunofluorescence staining and statistical graph of VE-cadherin expression in A2780 and CAOV3 tumors; n = 3 independent experiments per group. Scale bar: 10 μm. f Fluorescence staining and statistical graph of EdU incorporation in A2780 and CAOV3 tumors; n = 3 independent experiments per group. Scale bar: 100 μm. g Western blot and statistical graph of slug and snail expression in A2780 and CAOV3 tumors; n = 4 independent experiments per group. *p < 0.05, **p < 0.01, ***p < 0.001 for the indicated comparisons. The data are presented as the mean ± SEM values