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Fig. 2 | Cancer Cell International

Fig. 2

From: Establishment and characterization of HXWMF-1: the first mouse fibroblastic tumor cell line derived from leukemia-associated fibroblasts

Fig. 2

Growth curve of HXWMF-1 cells at different PDLs. Cells were cultured in a 6-well culture plate at 2 × 103/9.6 cm2 in RPMI-1640 medium with 10% FBS and grown for 8 days. Viable cells were counted using trypan blue staining every day. Experiments were performed in triplicate. a 100 PDLs. b 300 PDLs. c 500 PDLs. d DNA index showed DNA aneuploid of HXWMF-1. Cell cycle was analyzed by PI staining. DNA index was calculated as the ratio of aneuploid to diploid G0/G1 peak channel

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