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Fig. 3 | Cancer Cell International

Fig. 3

From: Establishment and characterization of HXWMF-1: the first mouse fibroblastic tumor cell line derived from leukemia-associated fibroblasts

Fig. 3

Authorization of HXWMF-1 cells. a STR of HXWMF-1 cells showed that HXWMF-1 cells were originated from mouse. b G-banding karyotype showed a complex chromosome aberration with total 60–70 chromosomes in HXWMF-1 cells. c Fibroblastic cell markers of HXWMF-1 cells. Cells were lysed, and extracts were analyzed by western blotting for α-SMA, FSP1, Vimentin, HSP47, FAP, PDGFRα and PDGFRβ. β-Actin was used as an internal control. Experiments were performed in triplicate. d Wound healing assay showed that HXWMF-1 cells closed the scratched area in 72 h

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