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Fig. 7 | Cancer Cell International

Fig. 7

From: Identifying SLC27A5 as a potential prognostic marker of hepatocellular carcinoma by weighted gene co-expression network analysis and in vitro assays

Fig. 7

GSEA and in vitro experiments in HepG2 cell line. The gene set of LIAO_METASTASIS (a) and Wnt/β-catenin pathway (b) was significantly enriched in the low SLC27A5 group. qPCR and western blot showed FATP5 (SLC27A5) in HepG2 was knocked down by small interference RNA (c). Both experiments were replicated three times. ** P < 0.01 by paired Student’s t test. The level of SOX2, MMP7, IL8 by qPCR and c-myc by western blot all increased after SLC27A5 knocked down (d). The tests were replicated three times, respectively. *P < 0.05 by paired Student's t test (e) Western blot assasys indicated that epithelial mesenchymal transition markers were altered after SLC27A5 knocked down. The assays were replicated at least twice. * P < 0.05 by paired Student’s t test. (f) The transwell migration assay showed increased migration ability of HepG2 cells treated with si-SLC27A5. Four fields were captured at 100 × magnification. **** P < 0.0001 by Student’s t test

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