Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: miR-34a mimic or pre-mir-34a, which is the better option for cancer therapy? KatoIII as a model to study miRNA action in human gastric cancer cells

Fig. 4

The effect of stable transfection of pre-mir-34a on target genes expression. a Sequence of DNA fragment, 248 base pairs in length, including pre-mir-34a (highlighted in grey) and flanking sequences was obtained from the human gene. The DNA fragment was cloned in the MCS between BglII and HindIII restriction sites. The RNA expression levels of miR-34a-5p, β-catenin and Notch1were quantified using qRT-PCR analysis. b The miR-34a-5p expression was significantly enhanced in KatoIII-pGFPC1-34a cells compared with the equivalent control cells, KatoIII-pGFPC1-empty (p < 0.05). c A non-significant mild reduction of β-catenin mRNA level was determined in KatoIII-pGFP-34a cells relative to the KatoIII-pGFPC1-empty cells. d The Notch1 mRNA level was significantly decreased in KatoIII-pGFP-34a cells relative to KatoIII-pGFPC1-empty cells (p < 0.01). e The expression level of β-catenin protein was detected using western blot analysis. The data demonstrated that the β-catenin protein expression was significantly reduced in KatoIII-pEGFPC1-34a cells relative to the control cells (p < 0.05)

Back to article page
\