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Fig. 3 | Cancer Cell International

Fig. 3

From: LINC00514 upregulates CCDC71L to promote cell proliferation, migration and invasion in triple‐negative breast cancer by sponging miR-6504-5p and miR-3139

Fig. 3

MiR-6504-5p and miR-3139 were the downstream miRNAs for LINC00514. a Subcellular fractionation assay determined LINC00514 location in TNBC cells. b StarBase predicted miRNAs binding to LINC00514. c RNA pull-down assay was utilized for validating the binding of LINC00514 to potential miRNAs. d The miR-6504-5p/miR-3139 binding sites for LINC00514 were predicted by starBase and their matched mutated sites were designed. e RT-qPCR analysis of miR-6504-5p/miR-3139 expression in MDA-MB-468 and HCC1937 cells transfected with miR-6504-5p/miR-3139 mimics. f Luciferase reporter LINC00514-WT/Mut was built to verify the binding of LINC00514 to miR-6504-5p/miR-3139. g RT-qPCR analysis of miR-6504-5p expression or miR-3139 expression in TNBC tissues and paired adjacent tissues. h Correlation analysis of LINC00514 expression and miR-6504-5p/miR-3139 expression in TNBC tissues. **p < 0.01, ***p < 0.001

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