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Fig. 3 | Cancer Cell International

Fig. 3

From: Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma

Fig. 3

MiR-488 was screened to be a target of circ_0000745. a The distribution of circ_0000745 in the cytoplasm or in the nucleus was distinguished. b The targets of circ_0000745 were obtained from starbase, circBANK and circinteractome. c The expression of four potential target miRNAs in Cal-27 and SCC9 cells after circ_0000745 knockdown was detected by qPCR. d The binding site between circ_0000745 and miR-488 and mutant binding site. e The efficiency of miR-488 mimic was examined using qPCR. The relationship between circ_0000745 and miR-488 was verified by (f) dual-luciferase reporter assay, (g) pull-down assay and (h) RIP assay. i Thde expression of miR-488 in HOK, Cal-27, SCC-25, SCC9 and HSC-3 cells was measured by qPCR. ***P < 0.001

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