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Fig. 4 | Cancer Cell International

Fig. 4

From: Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma

Fig. 4

Circ_0000745 downregulation inhibited OSCC development in vitro by increasing the level of miR-488. a The efficiency of miR-488 inhibitor was checked by qPCR. In Cal-27 and SCC9 cells transfected with sh-circ_0000745#1 + anti-NC, sh-NC + anti-NC or sh-circ_0000745#1 + anti-miR-488, cell proliferation was assessed by (b) CCK-8 assay and (c) colony formation assay. d Cell apoptosis was identified by flow cytometry assay. e, f Cell cycle distribution at different phases was monitored by flow cytometry assay. g The protein levels of PCNA, Cleaved-caspase 3 and CCND1 were quantified by western blot. *P < 0.05, **P < 0.01 and ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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