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Fig. 6 | Cancer Cell International

Fig. 6

From: Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma

Fig. 6

Circ_0000745 interacted with HuR to regulate the expression of CCND1. a The expression of CCND1 in HOK, Cal-27, SCC-25, SCC9 and HSC-3 cells was measured by western blot. bc The expression of CCND1 in OSCC tissues and matched normal tissues was measured by western blot and qPCR. d The correlation between circ_0000745 expression and CCND1 expression in OSCC tissues. e The RNA binding proteins of circ_0000745 were provided by circinteractome. f The effects of circ_0000745 dysregulation on the degradation of CCND1 were assessed using actinomycin D. g, h The interaction between circ_0000745 and HuR was determined by pull-down assay and RIP assay. ij The enrichment of CCND1 3′UTR was checked in Cal-27 and SCC9 cells with sh-NC or sh-circ_0000745#1 transfection incubated with HuR or IgG antibody was detected by qPCR. k The efficiency of HuR overexpression was checked by western blot. l, m The expression of CCND1 in Cal-27 and SCC9 cells transfected with sh-NC + vector, sh-circ_0000745 + vector or sh-circ_0000745 + HuR was measured by qPCR and western blot. **P < 0.01 and ***P < 0.001

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