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Fig. 2 | Cancer Cell International

Fig. 2

From: Targeting a novel LncRNA SNHG15/miR-451/c-Myc signaling cascade is effective to hamper the pathogenesis of breast cancer (BC) in vitro and in vivo

Fig. 2

The regulatory mechanisms of LncRNA SNHG15, miR-451 and c-Myc were investigated. The online starBase software (http://starbase.sysu.edu.cn/) was employed to predict the binding sites of miR-451 with (a) LncRNA SNHG15 and (d) 3′UTR of c-Myc mRNA. Dual luciferase reporter gene system was performed to validate the targeting sites of miR-451 with (b, c) LncRNA SNHG15 and (E, F) 3′UTR of c-Myc mRNA in BC cells. By performing Real-Time qPCR and Western Blot, we evidenced that (g–i) LncRNA SNHG15 positively regulated c-Myc, and (j–l) miR-451 negatively regulated c-Myc in BC cells. In addition, (mo) silencing of LncRNA SNHG15 inhibited c-Myc expressions through releasing miR-451. Each experiment repeated at least 3 times, and *P < 0.05 indicated statistical significance

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