Fig.3

BTK inhibitors induced CYLD dependent apoptosis in non-GCB-DLBCL. a, b OCI-Ly10 (a) and HBL-1 (b) cells were transfected with non-targeting or CYLD-targeting shRNA. Lysates were blotted with the indicated antibodies. c–f Control or CYLD-deficient OCI-Ly10 and HBL-1 cells were treated with DMSO or 10 μM PCI-32765 (c, d) or 10 μM ACP-196 (e, f) for 72-120 h, cell viability was assessed using CellTiter-Glo assay. g, h Control or CYLD-deficient OCI-Ly10 and HBL-1 cells were treated with DMSO or 10 μM PCI-32765 (g) or 10 μM ACP-196 (h) for 72 h, NFκB p65 subunit expression levels were measured by ELISA. i, j Control or CYLD-deficient OCI-Ly10 (i) and HBL-1 (j) cells were treated with DMSO or 10 μM PCI-32765 for 48 h, lysates were blotted with the indicated antibodies. The ATP level for each cell line treated with DMSO at each time point was set at 100%. The bars represent the mean ± SD from three independent experiments. **P < 0.01