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Fig. 2 | Cancer Cell International

Fig. 2

From: Macrophages-derived exosomal lncRNA LIFR-AS1 promotes osteosarcoma cell progression via miR-29a/NFIA axis

Fig. 2

LncRNA LIFR-AS1 is highly expressed in Mφ-CM co-cultured osteosarcoma cells and acts as a sponge for miR-29a. a Heatmap showing the differentially expressed miRNAs. b the expression of miR-29a was verified using qRT-PCR. c Heatmap representing the differentially expressed lncRNAs. d LIFR-AS1 was the only intersecting gene between predicted target lncRNAs of miR-29a and differentially expressed lncRNAs. e The expression of LIFR-AS1 was verified using qRT-PCR. f Schematic representation of the binding site between LIFR-AS1 and miR-29a. g The dual-luciferase reporter assay verified the direct binding between LIFR-AS1 and miR-29a. hj LIFR-AS1 was highly expressed and miR-29a was lowly expressed in osteosarcoma tissues, and negatively correlated. kl The expression of LIFR-AS1 and miR-29a was detected in osteosarcoma cell lines and hFOB. m ENCORI database revealed that there was a significant positive correlation between miR-29a expression and overall survival. n–o The expression of LIFR-AS1 and miR-29a in osteosarcoma cells. pq Overexpressed LIFR-AS1 can significantly promote proliferation and invasion of osteosarcoma cells, but miR-29a overexpression can rescue its effects. oe-LIFR-AS1: LIFR-AS1 overexpressed; oe-LIFR-AS1 + mimic NC: co-transfecting LIFR-AS1 overexpressed plasmid and miRNA mimics NC; oe-LIFR-AS1 + miR-29a mimic: co-transfecting LIFR-AS1 overexpressed plasmid and miR-29a mimic. *p < 0.05, **p < 0.01

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