Fig. 3
LIFR-AS1 can be transmitted from macrophages to osteosarcoma cells via exosomes. a Representative image of exosomes under TEM. b NTA results showed that Mφ-Exos were located predominately around 100 nm. c Exosome surface marker proteins CD9, CD63 was detected by western blot. d Macrophages-derived exosomes were ingested by 143B cells. e LIFR-AS1 was knocked down by siRNAs in both macrophages and Mφ-Exos. f The expression of LIFR-AS1 in Mφ-Exos co-cultured osteosarcoma cells. g The expression of miR-29a can be regulated by miR-29a mimics and inhibitors in osteosarcoma cells. h The expression of miR-29a was detected under different conditions. Mφsi−LIFR−AS1#1-Exo + miR-29a inhibitor/inhibitor NC: Cells were transfected with miR-29a inhibitor/inhibitor NC and co-cultured with LIFR-AS1- knockdown Mφ-Exos. *p < 0.05, ns = no significant