Fig. 4
Macrophages-derived exosomal LIFR-AS1 promotes osteosarcoma cell progression via sponging miR-29a. a The cck-8 assay for cell proliferation. b Colony formation assay. c Flow cytometry for apoptosis. d Wound-healing assay. e Transwell assays for migration and invasion. f Statistical results of apoptosis, wound-healing, and transwell assays are showing accordingly. g The expression levels of apoptosis-related and EMT-related proteins were detected by western blot. Mφsi−LIFR−AS1#1-Exo + miR-29a inhibitor: Cells were transfected with miR-29a inhibitor/inhibitor NC and co-cultured with LIFR-AS1- knockdown Mφ-Exos. *p < 0.05, **p < 0.01, compared with control group; #p < 0.05, ##p < 0.01, compared with Mφ-Exo group; &p < 0.05, &&p < 0.01, compared with Mφsi−LIFR−AS1#1-Exo group