Fig. 5

NFIA is the direct target gene of miR-29a. a Venn diagram of the predicted targeting genes of miR-29a. b–c The expression of NFIA protein was detected in osteosarcoma cell lines and human osteoblast cell Line hFOB. d schematic representation of NFIA 3′-UTR containing wild-type or mutant binding site for miR-29a. e The dual-luciferase reporter assay verified the direct binding between NFIA and miR-29a. f Western blot confirmed that NFIA protein was downregulated by miR-29a mimics in osteosarcoma cells. **p < 0.01