Fig. 7

Validation of the TME regulating, Oncogenic promoting and immune checkpoint associating role of CALD1 in the GEO cohort. a Kaplan-Meier curve validated the difference of OS between high and low CALD1 expression BLCA Patients in the GEO cohort, with a P-value < 0.001. The AUC value of the ROC curve for CALD1 prediction of tumor progression in the GEO cohort was 0.730. A significantly higher level of CALD1 was also detected in patients with higher grade, stage, T and N classification in the GEO cohort. b-c CAFs (p < 0.001), macrophage (p < 0.001), stromal score (p < 0.001), immune score (p < 0.001) and ESTIMATE score (p < 0.001) were significantly different between the high and low CALD1 expression groups, and their expression level was also strongly correlated with CALD1(CAFs: R = 0.86, p<0.001; macrophage: R = 0.47, p < 0.0011; stromal score: R = 0.72, p< 0.001; immune score: R = 0.51, p< 0.001; stromal score: R = 0.81, p<0.001). d-e Close relationships were validated between CALD1 and immune checkpoint related genes, including CTLA4 (p < 0.001, R = 0.46), GAL9 (p<0.05, R=−0.30), LAG3 (p<0.001, R = 0.31), PD-L2 (p<0.001, R = 0.28) and TIM3 (p<0.001, R = 0.38) in the GEO cohort