Fig. 4

EZH2-dependent SOX9 expression regulates the activation of NF-κB signaling. a LNCaP cells were transfected with indicated siRNAs for 36 h, the relative mRNA expression levels of SOX9 were determined by real-time PCR assay. *P < 0.05, **P < 0.01. b LNCaP cells were treated with 10 μM EPZ6438 or GSK126 for 24 h. The relative mRNA levels of SOX9 were then determined. *P < 0.05. c LNCaP cells were transfected with indicated siRNAs for 12 h, then co-transfected with pGL3-NFkB-Luc and pSV40-renilla plasmids for 24 h. The luciferase activity was then measured. **P < 0.01. d LNCaP cells were co-transfected with indicated plasmids for 24 h. The luciferase activity was then measured. **P < 0.01. e LNCaP cells were transfected with indicated siRNAs for 24 h and then treated with or without 10 μM EPZ6438 or GSK126 for additional 24 h. The luciferase activity was then measured. **P < 0.05. f LNCaP cells were transfected with indicated siRNAs for 24 h and then treated with or without 10 μM EPZ6438 for additional 24 h. CCK-8 was then added and the absorbance at A450 was determined by a microplate reader. **P < 0.01