Fig. 6

Silencing of TNFRSF11A rendered PCa cells to EZH2 inhibitors treatment. a LNCaP cells were treated with 10 μM EPZ6438 or GSK126 for 24 h. The relative mRNA levels of TNFRSF11A were then determined. **P < 0.01, ***P < 0.001. b LNCaP cells were transfected with indicated siRNAs for 36 h, the relative mRNA expression levels of TNFRSF11A were determined. ***P < 0.001. c LNCaP cells were transfected with indicated siRNAs for 12 h, then co-transfected with pGL3-NFkB-Luc and pSV40-renilla plasmids for 12 h and treated with or with EPZ6438 for additional 24 h. The luciferase activity was then measured. ***P < 0.001. d LNCaP cells were transfected with indicated siRNAs for 12 h and treated with or with EPZ6438 for additional 24 h. CCK-8 was then added and the absorbance at A450 was determined by a microplate reader. **P < 0.01