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Fig. 4 | Cancer Cell International

Fig. 4

From: Morphological and molecular characteristics of spheroid formation in HT-29 and Caco-2 colorectal cancer cell lines

Fig. 4

Serial spheroid formation capacity of colonospheres. To evaluate the self-renewal properties of generated spheroids, secondary spheroid formation was performed by serial passaging. Cells derived from a–f HT-29 and e–h Caco-2 spheroids generated spheroids for four consequence passage (P1–P4). There were no obvious differences in size and morphology of spheroids in different passages and their derived cells maintain their self-renewal capacity over several passages

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